Pierce Bca Protein Assay Protocol

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The Pierce Protein Assay is a method of protein quantification. It provides quick estimation of the protein amount in a given sample.

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Maps, Directions, and Place Reviews

Protocol

The assay is separated into three main parts:

1. Preparation of the Diluted Albumin (BSA) Standards,
2. Preparation of the bicinchoninic acid (BCA) working reagent,
3. Quantification of proteins (using either test tube or microplate procedure)

Pierce Bca Protein Assay Protocol Video

Advantages and disadvantages

Advantages

1. The method is able to detect as low as 25 µg/ml and up to 2000 µg/ml of protein in a 65 ul sample, using standard protocol.
2. The method may be preferred for samples containing detergents or other reducing agents.
3. The method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford) Assays.
4. The method has a stable end point.

Disadvantages

1. The method has greater protein-to-protein variability than the BCA Assay.

Source of the article : Wikipedia