Protein methylation is the process through which a group of specific enzymes, the methyltransferases modify proteins by adding a methyl groups. It occurs on the nitrogen side-chains in arginine and lysine residues and the carboxy-termini of a few different proteins. Methylation occurring on nitrogen atoms in N-terminals usually cannot be reversed and creates new amino acid residues. Methylations on the C-terminus can increase a protein´s chemical repertoire and are known to have a major effect on the functions of a protein
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Background
Protein methylation is the addition a methyl group to proteins, typically on the arginine or lysine amino acid residues in the protein sequence. Before methylation occurs at these termini additional processing may be required. Regions of methylated proteins are usually glycine and arginine-rich and are referred to as "GAR motifs".
In biology, methyltransferases catalyze the process, activated primarily by S-adenosylmethionine. Protein methylation has been most studied in the histones, where the transfer of methyl groups from S-adenosyl methionine is catalyzed by histone methyltransferases. Histones that are methylated on certain residues can act epigenetically to repress or activate gene expression. Protein methylation is one type of post-translational modification. Methylation occurring on nitrogen atoms in N-terminals usually cannot be reversed and creates new amino acid residues.
Protein Methylation Video
Methylation by protein
Arginine
Arginine methylation is a posttranslational modification; it usually occurs in the nucleus and the main pool of modified proteins have RNA binding properties. Methylation of arginine residues is catalyzed by at least two different classes of protein arginine methyltransferases (PRMTs): Arginine can be methylated once (monomethylated arginine) or twice. Type I enzymes catalyze with either both methyl groups on one terminal nitrogen the asymmetric dimethylarginine (N G,N G-dimethylarginine). Type II enzyme catalyzes formation of symmetric dimethylarginine with one methyl group on each nitrogens /symmetric N G,N' G-dimethylarginine residues. Both types generate N G-monomethylarginine intermediates.
Enzymes that facilitate histone acetylation and histones are arginine methylated. Arginine methylation affects the interactions between proteins and has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Lysine
Lysine can be methylated once, twice, or three times by lysine methyltransferases. Most lysine methyltransferases contain an evolutionarily conserved SET domain, which possess S-adenosylmethionine-dependent methyltransferase activity, but are structurally distinct from other S-adenosylmethionine binding proteins. Lysine methylation plays a central part how histones interact with proteins. Lysine residues accept up to three methyl groups forming mono-, di-and trimethylated derivatives.
Different SET domain-containing proteins possess distinct substrate specificities. For example, SET1, SET7 and MLL methylate lysine 4 of histone H3, whereas Suv39h1, ESET and G9a specifically methylate lysine 9 of histone H3. Methylation at lysine 4 and lysine 9 are mutually exclusive and the consequences of site-specific methylation are diametrically opposed. Methylation at lysine 4 correlates with an active state of transcription. Methylation at lysine 9 is associated with transcriptional repression and heterochromatin. Other lysine residues on histone H3 and histone H4 are also important sites of methylation by specific SET domain-containing enzymes. Although the histones are the prime target of lysine methyltransferases, other cellular proteins carry N-methyllysine residues including elongation factor 1A and the calcium sensing protein calmodulin.
Prenylcysteine
Eukaryotic proteins with C-termini that end in a CAAX motif are often subjected to a series of posttranslational modifications. The CAAX-tail processing takes place in three steps: First, a prenyl lipid anchor is attached to the cysteine through a thioester linkage. Then endoproteolysis occurs to remove the last three amino acids of the protein to expose the prenylcysteine ?-COOH group. Finally, the exposed prenylcysteine group is methylated. The importance of this modification can be seen in targeted disruption of the methyltransferase for mouse CAAX proteins, when isoprenylcysteine carboxyl methyltransferase resulted in mid-gestation lethality.
The biological function of prenylcysteine methylation is to facilitate the targeting of CAAX proteins to membrane surfaces within cells. Prenylcysteine can be demethylated and this reverse reaction is catalyzed by isoprenylcysteine carboxyl methylesterases. CAAX box containing proteins that are prenylcysteine methylated include Ras, GTP-binding proteins, nuclear lamins and certain protein kinases. Many of these proteins participate in cell signaling, and they utilize prenylcysteine methylation to concentrate them on the cytosolic surface of the plasma membrane where they are functional.
Protein phosphatase 2
In eukaryotic cells, phosphatases catalyze the removal of phosphate groups from tyrosine, serine and threonine phosphoproteins. The catalytic subunit of the major serine/threonine phosphatases, like Protein phosphatase 2 is covalently modified by the reversible methylation of its C-terminus to form a leucine carboxy methyl ester. Unlike CAAX motif methylation, no C-terminal processing is required to facilitate methylation. This C-terminal methylation event regulates the recruitment of regulatory proteins into complexes through the stimulation of protein-protein interactions, thus indirectly regulating the activity of the serine-threonine phosphatases complex. Methylation is catalyzed by a unique protein phosphatase methyltransferase. The methyl group is removed by a specific protein phosphatase methylesterase. These two opposed enzymes make serine-threonine phosphatases methylation a dynamic process in response to stimuli.
L-isoaspartyl
Damaged proteins accumulate isoaspartyl which causes protein instability, loss of biological activity and stimulation of autoimmune responses. A methyltransferase dependent pathway exists for the conversion of L-isoaspartyl back to l-aspartyl. The spontaneous age-dependent degradation of l-aspartyl residue results in the formation of a succinimidyl intermediate, a succinimide radical. This is spontaneously hydrolyzed either back to l-aspartyl or, in a more favorable reaction, to abnormal L-isoaspartyl. To prevent the accumulation of L-isoaspartyl, this residue is methylated by the protein L-isoaspartyl methyltransferase, which catalyzes the formation of a methyl ester, which in turn is converted back to a succinimidyl intermediate. Loss and gain of function mutations have unmasked the biological importance of the L-isoaspartyl O-methyltransferase in age-related processes: Mice lacking the enzyme die young of fatal epilepsy, whereas flies engineered to over-express it have an increase in life span of over 30%.
Physical effects
A common theme with methylated proteins, as with phosphorylated proteins, is the role this modification plays in the regulation of protein-protein interactions. The arginine methylation of proteins can either inhibit or promote protein-protein interactions depending on the type of methylation. The asymmetric dimethylation of arginine residues in close proximity to proline-rich motifs can inhibit the binding to SH3 domains. The opposite effect is seen with interactions between the survival of motor neurons protein and the snRNP proteins SmD1, SmD3 and SmB/B', where binding is promoted by symmetric dimethylation of arginine residues in the snRNP proteins.
A well-characterized example of a methylation dependent protein-protein interaction is related to the selective methylation of lysine 9, by SUV39H1 on the N-terminal tail of the histone H3. Di- and tri-methylation of this lysine residue facilitates the binding of heterochromatin protein 1 (HP1). Because HP1 and Suv39h1 interact, it is thought the binding of HP1 to histone H3 is maintained and even allowed that to spread along the chromatin. The HP1 protein harbors a chromodomain which is responsible for the methyl-dependent interaction between it and lysine 9 of histone H3. It is likely that additional chromodomain-containing proteins will bind the same site as HP1, and to other lysine methylated positions on histones H3 and Histone H4.
C-terminal protein methylation regulates the assembly of protein phosphatase. Methylation of the protein phosphatase 2A catalytic subunit enhances the binding of the regulatory B subunit and facilitates holoenzyme assembly.
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