Coomassie Plus Protein Assay Reagent Protocol

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The Pierce Protein Assay is a method of protein quantification. It provides quick estimation of the protein amount in a given sample.

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Maps, Directions, and Place Reviews

Protocol

The assay is separated into three main parts:

1. Preparation of the Diluted Albumin (BSA) Standards,
2. Preparation of the bicinchoninic acid (BCA) working reagent,
3. Quantification of proteins (using either test tube or microplate procedure)

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Advantages and disadvantages

Advantages

1. The method is able to detect as low as 25 µg/ml and up to 2000 µg/ml of protein in a 65 ul sample, using standard protocol.
2. The method may be preferred for samples containing detergents or other reducing agents.
3. The method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford) Assays.
4. The method has a stable end point.

Disadvantages

1. The method has greater protein-to-protein variability than the BCA Assay.

Source of the article : Wikipedia